资源类型

期刊论文 1

年份

2016 1

关键词

检索范围：

排序： 展示方式：

Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

《化学科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 238-244 doi: 10.1007/s11705-016-1566-2

摘要： MATTt (a thermostable methionine adenosyltransferase from HB27) was overexpressed in and purified using Ni-NTA affinity column. The enzymatic activity of MATTt was investigated in a temperature range from 30 °C to 90 °C, showing that MATTt exhibited a high enzymatic activity and good thermostability at 80 °C. Circular dichroism spectra reveals that MATTt contains high portion of -sheet structures contributing to the thermostability of MATTt. The kinetic parameter, is 4.19 mmol/L and 1.2 mmol/L for ATP and methionine, respectively. MATTt exhibits the highest enzymatic activity at pH 8. Cobalt (Co ) and zinc ion (Zn ) enhances remarkably the activity of MATTt compared to the magnesium ion (Mg ). All these results indicated that the thermostable MATTt has great potential for industry applications.

关键词： ion-preference     methionine adenosyltransferase     secondary structure     thermostability     Thermus thermophilus    

HTML PDF 收藏

标题 作者 时间 类型 操作

Functional characterization of a thermostable methionine adenosyltransferase from

Yanhui Liu,Biqiang Chen,Zheng Wang,Luo Liu,Tianwei Tan

期刊论文